ACTIVITY AND ISOZYME COMPOSITION OF PEROXIDASE IN SCOTS PINE (PINUS SYLVESTRIS L.) NEEDLES EFFECTED BY TECHNOGENIC EMISSIONS FROM VARIOUS ENTERPRISES AND VEHICLES

Background. The technogenic pollution leads to excessive production of reactive oxygen species (ROS) in plants which are highly and toxic cause damage biomolecules. Plants have a complex antioxidant defense system that protects cells from the ROS maintain homeostasis. most important link this is enzymes, particular, peroxidase. It was interest determine expression protective properties one sensitive coniferous under influence emissions various enterprises vehicles.
 Purpose. Investigation activity isoenzyme composition peroxidase needles Pinus sylvestris L. different compositions Baikal region.
 Materials methods. pine were collected on sample plots located near an aluminum plant, thermal power chemical coal mining enterprise, highway. soluble guaiacol-dependent peroxidases defined by spectrophotometry reaction mixture with citrate-phosphate buffer, hydrogen peroxide, guaiacol. Native polyacrylamide gel electrophoresis used for determination isoforms.
 Results. shown increase total ranged 6 22 times polluted areas. Maximum enzyme found needle samples smelter, whose characterized large amounts fluorides polycyclic aromatic hydrocarbons. high variability isoform Scots industrial revealed. expressed emergence new isoforms zone fast-moving (Rf 61 100) medium-moving 31 60) items. maximum number (nine) smelter only two ones detected background area.
 Conclusion. Peroxidase its newly formed can adequately reflect degree trees decline. indicators also be monitoring forests condition.